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mcherry sephluorin vector  (Addgene inc)


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    Structured Review

    Addgene inc mcherry sephluorin vector
    Mcherry Sephluorin Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+sephluorin+vector/mCherry-SEpHluorin+(Plasmid+%2332001)/pmc06776657-95-14-16
    Average 94 stars, based on 27 article reviews
    mcherry sephluorin vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish
    Article Snippet: .. Full length mouse otoferlin (denoted as FL, amino acids 1–1992) was cloned into the mCherry-SEpHluorin vector (Addgene plasmid # 32001). ..

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..

    Plasmid Preparation:

    Article Title: Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish
    Article Snippet: .. Full length mouse otoferlin (denoted as FL, amino acids 1–1992) was cloned into the mCherry-SEpHluorin vector (Addgene plasmid # 32001). ..

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..

    Amplification:

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..

    Polymerase Chain Reaction:

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..

    Sequencing:

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..

    Generated:

    Article Title: Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
    Article Snippet: .. To generate UAS-myr-mCherry-pHluorin , we amplified DNA encoding the first 30 amino acids of Drosophila Src64B from P[UAS-myr-RFP] (donated from Henry Chang) and PCR amplified the mCherry-pHluorin sequence from the mCherry-SEpHluorin vector (addgene, plasmid #32001, donated by Sergio Grinstein), generated the final linear DNA 5′- NotI-myr-linker-mCherry-pHluorin-XhoI -3′ by overlap extension PCR, then cloned it into the pUASTattB vector. ..



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    94
    Addgene inc mcherry sephluorin vector
    Mcherry Sephluorin Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+sephluorin+vector/mCherry-SEpHluorin+(Plasmid+%2332001)/pmc06776657-95-14-16
    Average 94 stars, based on 1 article reviews
    mcherry sephluorin vector - by Bioz Stars, 2026-10
    94/100 stars
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